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采用细胞色素c法研究了水杨酸铜分别在中性、正负电性脂质体中SOD样活性的变化情况。证明中性脂质体包裹的水杨酸铜的SOD样活性比水杨酸铜在水溶液中提高了3倍多。但正负电性脂质体包裹的水杨酸铜的SOD样活性降低。并发现在实验浓度范围内,负电性脂质体包裹的水杨酸铜,活性变化无规则,且荷负电量多的脂质体包裹的水杨酸铜促进超氧自由基的歧化,而荷负电量少的脂质体仅在低浓度区对超氧的歧化表现为促进作用。
Cytochrome c assay was used to study the changes of SOD-like activity in neutral, positive and negative liposomes of salicylic acid respectively. It was demonstrated that the SOD-like activity of neutral liposome-encapsulated salicylic acid copper was more than three-fold higher than that of copper salicylate in aqueous solution. However, the SOD-like activity of copper salicylate encapsulated by the electropositive liposomes decreased. It was found that in the experimental concentration range, negatively charged liposomes encapsulated salicylic acid copper showed no regular changes in activity and the liposomal copper salicylate encapsulated in the negatively charged liposomes promoted the disproportionation of superoxide radicals. The negatively charged The low amount of liposomes only promoted the disproportionation of superoxide in the low concentration area.