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目的了解慢性粒细胞白血病(chronic myeloid leukemia,CML)患者慢性期(chronic phase,CP)和缓解期(complete remission,CR)外周血T细胞受体(T cell receptor,TCR)Vδ亚家族T细胞的分布和克隆性情况。方法利用RT-PCR扩增8例CML-CP和8例CML-CR患者外周血T细胞中8个Vδ亚家族基因的互补决定区(CDR3),了解TCR Vδ亚家族T细胞的分布和利用情况;阳性产物进一步经基因扫描分析CDR3长度,从而了解其克隆性。10例健康人外周血作为对照。结果8例CML-CP患者外周血T细胞平均表达(3.25±0.89)个Vδ亚家族,主要以Vδ1、Vδ2和Vδ3的表达为主,而8例CR期患者则平均表达(3.50±0.76)个Vδ亚家族,主要集中在Vδ1、Vδ2、Vδ3和Vδ8中表达;两组与健康对照组(3.50±0.52)比较无统计学意义。此外,Vδ8表达频率在CML-CR患者(5/8)中略高于健康人(3/10)和CML-CP患者(2/8)。基因扫描绝大多数患者和健康人外周血TCR Vδ亚家族T细胞均存在克隆性增殖现象,主要以Vδ1-Vδ3为主,但在CML-CR患者中,表达Vδ7亚家族的两例样本均呈克隆性增殖,这在10例健康人中均未检测到。结论3组样本的外周血T细胞中TCR Vδ谱系和克隆性增殖类似,提示CML患者T细胞免疫抑制可能并不主要累及TCR Vδ谱系变化,并在CML-CR期所出现了一些不同的Vδ表达和克隆模式。
Objective To investigate the expression of T cell receptor (TCR) Vδ subfamily T cells in peripheral blood of chronic myeloid leukemia (CML) patients with chronic phase (CP) and complete remission (CR) Distribution and cloning of the situation. Methods The complementarity determining regions (CDRs) of 8 Vδ subfamilies in peripheral blood T cells of 8 CML-CP and 8 CML-CR patients were amplified by RT-PCR. The distribution and utilization of TCR Vδ subfamily T cells ; The positive product was further analyzed for CDR3 length by gene scanning to understand its clonality. 10 healthy people as the control of peripheral blood. Results The average number of T cells in peripheral blood of 8 patients with CML-CP was (3.25 ± 0.89) Vδ subfamilies, mainly Vδ1, Vδ2 and Vδ3, while the average of 8 patients with CR was 3.50 ± 0.76 Vδ subfamily, mainly in Vδ1, Vδ2, Vδ3 and Vδ8 expression; two groups and the healthy control group (3.50 ± 0.52) no statistical significance. In addition, the frequency of Vδ8 expression was slightly higher in CML-CR patients (5/8) than in healthy people (3/10) and CML-CP patients (2/8). Clonal proliferation of TCR Vδ subfamily T cells in the peripheral blood of most patients and healthy people was observed mainly in Vδ1-Vδ3. However, in CML-CR patients, two cases of Vδ7 subfamilies were expressed Clonal proliferation, which was not detected in 10 healthy subjects. Conclusions TCR Vδ pedigree and clonal proliferation in peripheral blood T cells in three groups are similar, suggesting that T cell immunosuppression in CML patients may not mainly affect the change of TCR Vδ lineage and some different Vδ expression in CML-CR stage And clone mode.