近几年来,植物基因转移的方法大致可分为两类:一类是直接导入法;另一类是通过一种经遗传改造的土壤农杆菌T4质粒以及病毒作为载体,来实现植物细胞的转化。我国自七十年代开始,大多采用基因直接导入法。诸如DNA涂抹柱头、DNA显微注射等便属此法。为了探索外源DNA导入水稻中较为 In recent years, plant gene transfer methods can be broadly divided into two categories: one is the direct introduction method; the other is through a genetically modified Agrobacterium tumefaciens T4 plasmid and virus as a carrier, to achieve plant cell transformation . Since the seventies in our country, most of them have adopted gene direct introduction method. Such as DNA smear stigma, DNA microinjection and so on this method. In order to explore the introduction of foreign DNA more in rice