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目的 :探讨凋亡抑制蛋白survivin的表达与胰腺癌细胞增殖及其生物学特性的关系。方法 :用免疫组织化学方法Envision法对 5 1例胰腺导管癌survivin蛋白和ki6 7的表达进行检测。结果 :5 1例胰腺导管癌中survivin .蛋白的表达率为 80 .4 % ,ki6 7平均标记指数为 2 6 .5 2± 12 .2 2 ;二者在 11例癌旁非肿瘤胰腺组织中均未发现阳性表达。survivin的表达与组织学分级有关 (P <0 .0 1) ,而与临床分期和淋巴结转移关系不大 (P >0 .0 5 ) ;ki6 7标记指数与组织学分级和临床分期和淋巴结转移有关 (P <0 .0 1) ;survivin的阳性表达与肿瘤细胞的增殖指数密切相关 (P <0 .0 1)。结论 :survivin基因在胰腺癌中高度表达可能与肿瘤的发生发展及细胞的增殖密切相关。
Objective: To investigate the relationship between the expression of survivin and the proliferation and biological characteristics of pancreatic cancer cells. Methods: Envision method was used to detect the expression of survivin protein and ki6 7 in 51 cases of pancreatic ductal carcinoma. Results: The expression of survivin protein in 80 pancreatic ductal carcinomas was 80.4% and the average labeling index of ki6 7 was 26.252 ± 12.22 in 11 cases of pancreatic ductal carcinoma No positive expression was found. The expression of survivin was correlated with the histological grade (P <0.01), but not with clinical stage and lymph node metastasis (P> 0.05). The expression of survivin was correlated with histological grade, clinical stage and lymph node metastasis (P <0.01). The positive expression of survivin was closely related to the proliferation index of tumor cells (P <0.01). Conclusion: The high expression of survivin gene in pancreatic cancer may be closely related to tumorigenesis and cell proliferation.