论文部分内容阅读
AIM:to determine whether the gastrin stimulated intestinalcrypt cell(IEC-6)proliferation by induction of ornithinedecarboxylase(ODC).METHODS:IEC-6 cells were grown in DMEM containing50mL·L~(-1)dialyzed fetal bovine serum for 24h and then weretreated with gastrin.The proliferative capability of the cellswas monitored subsequently on d 1,2,3,and 4 aftertreatment with MTT assay at aborbance 570nm.The cellularODC mRNA expression,ODC activity,and putrescinecontent were examined by RT-PCR method,radiometrictechnique and high-performance liquid chromatography(HPLC)analysis respectively after 12h of treatment.RESULTS:On d1 after exposure of IEC-6 cells topentagastrin,the proliferation increased initially and reacheda peak on d3 at 250μg·L~(-1)concentration.Pentagastrin 500μg·L~(-1)increased cell proliferation on day 1 and day 2,andthen decreased,Compared with control group,pentagastrin250μg·L~(-1)increased ODC mRNA level by 1.09-fold(P<0.05),ODC activity by 1.71-fold(P<0.0]),and putrescinecontent 5.30-fold(P<0.01)respectively.Similarly,pentagastrin of 500μg·L~(-1)also increased ODC mRNA levelby 1.16-fold(P<0.05),ODC activity 1.63-fold(P<0.05),and putrescine content 4.4t-fold(P<0.01)respectively.But there was not significant difference between them.CONCLUSION:Gastrin is an agent which promotes IEC-6 cellproliferation involved in regulating ODC activity mechanism.
AIM: to determine whether the gastrin stimulated intestinal crypt cell (IEC-6) proliferation by induction of ornithine decarboxylase (ODC) .METHODS: IEC-6 cells were grown in DMEM containing 50 mL·L -1 dialyzed fetal bovine serum for 24h and then weretreated with gastrin. The proliferative capability of the cells was taken by d 1,2,3 and 4 aftertreatment with MTT assay at aborbance 570 nm. The cellular ODC mRNA expression, ODC activity, and putrescine content were examined by RT-PCR method, radiometrictechnique and High-performance liquid chromatography (HPLC) analysis respectively after 12h of treatment. RESULTS: On d1 after exposure of IEC-6 cells topentagastrin, the proliferation increased initially and reached peak at d3 at 250μg · L -1 concentration. Pentagastrin 500μg ODC mRNA increased by 1.09-fold (P <0.05), ODC activity increased by 50% compared with that of control group 1.71-fold (P <0.0]), and putresc ODC mRNA levelby 1.16-fold (P <0.05), ODC activity was 1.63-fold (P <0.05), and putrescine content 4.4t-fold (P <0.01) respectively.But there was no significant difference between them. CONCLUSION: Gastrin is an agent which promotes IEC-6 cellproliferation involved in regulating ODC activity mechanism.