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彩色豆马勃(Pisolithus tinctorius(Pers.)Cok.& Couch是外生菌根菌之一~([1]),国外利用较多。在干旱瘠薄地区能提高寄主抗高温和抗旱力,并能刺激植株生长~([1])。一、子实体分离采取彩色豆马勃的担子果~([3]),选择无病虫危害,包被完整的,在无菌条件下进行分离培养,先用无菌水洗净,勾取富含孢子的子实体小块置于琼脂斜面。采用5种培养基:(1)经Modess修改的Hagem培养基~([4]),(2)Morkans修改的Melin培养基~([5]),(3)Lutz培养基,(4)Littman培养基,(5)土豆——葡萄糖培养基(P.G.A.)。
Pokotava tinctorius (Pers.) Cok. & Couch is one of the ectomycorrhizal fungi ([1]), more used abroad. In arid infertile areas can improve the host high temperature and drought resistance, and can Stimulation of plant growth ~ ([1]). First, the fruiting body to take the color beans red bean burl burden ~ ([3]), choose no pests and diseases harm, the package is complete, under sterile conditions for isolation and culture, First, sterile water was used to wash the sporulated fruiting bodies and placed on agar slats using five kinds of medium: (1) Modes modified Hagem medium ~ ([4]), (2) Morkans Modified Melin Medium ~ ([5]), (3) Lutz Medium, (4) Littman Medium, (5) Potato-Glucose Medium (PGA).