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为了使白地霉固定化小球处理太原市地下岩溶水达到最佳絮凝效果,分别研究了白地霉菌体的最佳扩大培养条件.同时,分别选用海藻酸钠(SA)、聚乙烯醇-海藻酸钠(PVA-SA)及聚乙烯醇-海藻酸钠-明胶(PVA-SA-明胶)作为固定化剂,固定化白地霉菌体,进行絮凝性能分析.最后,确定了固定化小球处理岩溶水的最佳搅拌时间及投加量,并与六水合氯化铝、自制三元复合吸附剂及壳聚糖的5%冰醋酸溶液的絮凝效果进行了比较.结果表明,将白地霉种子培养基按3%的接种量接入白地霉扩大培养基3中进行扩大培养,可在第2d获取活性较好的大量白地霉菌体进行固定化.处理岩溶水时应选用PVA-SA-明胶固定化的白地霉小球,并在其投加量为4g·L-1、搅拌时间为60min时,对岩溶水矿化度、硬度的最大絮凝率分别为96.88%、50.00%.经与化学混凝剂絮凝性能对比发现,微生物絮凝剂具有操作简便、无二次污染、絮凝效果较好的优点.
In order to optimize the flocculation effect of the white mildew-immobilized pellets on the underground karst water in Taiyuan City, the best expansion culture conditions of the white mildew molds were studied respectively.At the same time, sodium alginate (SA), polyvinyl alcohol-alginic acid (PVA-SA) and polyvinyl alcohol-sodium alginate-gelatin (PVA-SA-gelatin) were used as immobilizers to immobilize the fungus, and the flocculation performance was analyzed.Finally, the effect of immobilized pellets on karst water The best mixing time and dosage were compared with the flocculation effect of aluminum chloride hexahydrate, self-made ternary complex adsorbent and chitosan 5% glacial acetic acid solution.The results showed that the gelatin solution of Geotrichum candidum seed medium Inoculation of 3% inoculation access to expand the culture medium to extend the culture medium 3, can be obtained in the second day to take a good activity of a large number of white mold fungi immobilized when processing karst water should be selected PVA-SA-gelatin immobilized The maximum flocculation rate of the mineralization degree and the hardness of karst water were 96.88% and 50.00% respectively when the dosage was 4g · L-1 and the mixing time was 60min.After mixing with chemical coagulant Flocculation performance comparison found that microbial flocculant is easy to operate, no two Contamination, better flocculation advantages.