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糖尿病如控制不佳,可引起诸多并发症,其中认知功能障碍为其中之一,高压氧疗可改善神经功能障碍,本文通过观察对糖尿病大鼠行高压氧疗后其认知功能障碍的改变来探讨高压氧疗对糖尿病认知功能障碍的作用,为临床治疗提供思路。目的:随着生活水平提高,糖尿病发病率逐年升高,并有年轻化趋势,为社会带来经济负担外,患者生活质量逐年下降,其中认知功能障碍是糖尿病并发症之一,主要表现为学习能力、记忆力下降。高压氧疗具有促进神经功能修复,目前广泛运用于脑血管意外,热射病等疾病所致神经功能障碍患者脑神经功能恢复。本文探讨高压氧疗对糖尿病大鼠认知功能障碍的影响。方法:将60只成年SD大鼠(体重110g~130g),随机分为4组,正常对照组,高压氧疗组,糖尿病组,糖尿病高压氧疗组,每组15只。糖尿病组及糖尿病高压氧疗组均采用一次性腹腔内注射链脲佐菌素60mg/kg制备糖尿病模型,各大鼠72小时后测尾静脉血糖,若血糖≥16.9mmol/L,则纳入实验。标本处理及检测:造模成功后每组大鼠每日监测血糖及体重,正常对照组及糖尿病组予以正常饲养,不干涉其任何活动。高压氧疗组及糖尿病高压氧疗组除正常饲养外每日给予高压氧疗2小时,连续12周。12周后各组大鼠进行Morris水迷宫实验,连续5天,每日行定位航行实验,并记录逃避潜伏期。统计学处理:采用SPSS 13.0中方差分析和独立样本t检验进行统计学分析。所有数据均以均数±标准差(X±S)表示,统计结果以P<0.05和P<0.01作为差异有统计学意义的标准。
If the poor control of diabetes can cause a lot of complications, of which cognitive dysfunction is one of them, hyperbaric oxygen therapy can improve neurological dysfunction.This article through the observation of diabetic rats after hyperbaric oxygen therapy changes in cognitive dysfunction To investigate the role of hyperbaric oxygen therapy in diabetic cognitive dysfunction and to provide ideas for clinical treatment. OBJECTIVE: With the improvement of living standards, the incidence of diabetes is increasing year by year, with the tendency of becoming younger, bringing economic burden to the society, the quality of life of patients declines year by year. The cognitive dysfunction is one of the complications of diabetes, which is mainly manifested as Learning ability, memory loss. Hyperbaric oxygen therapy can promote the repair of nerve function, and is widely used in cerebrovascular accident, fever and other diseases caused by neurological dysfunction in patients with neurological functional recovery. This article discusses the impact of hyperbaric oxygen therapy on cognitive dysfunction in diabetic rats. Methods: Sixty adult SD rats weighing 110g ~ 130g were randomly divided into 4 groups: normal control group, hyperbaric oxygen therapy group, diabetic group and diabetic hyperbaric oxygen therapy group, with 15 rats in each group. Diabetes mellitus and diabetic hyperbaric oxygen therapy group were given a single intraperitoneal injection of streptozotocin 60mg / kg preparation of diabetic model, 72 hours after the test of tail vein blood glucose, blood glucose ≥ 16.9mmol / L, then included in the experiment. Specimens were processed and tested: blood glucose and body weight were monitored daily for each group of rats after successful modeling. Normal control group and diabetic group were reared normally and did not interfere in any activities. Hyperbaric oxygen therapy group and diabetic hyperbaric oxygen therapy group were given hyperbaric oxygen therapy for 2 hours daily for 12 weeks in addition to normal feeding. After 12 weeks, the rats in each group were subjected to Morris water maze test. For 5 consecutive days, their navigation experiments were conducted daily and the escape latency was recorded. Statistical analysis: SPSS 13.0 analysis of variance and independent samples t test for statistical analysis. All data were expressed as mean ± standard deviation (X ± S), the statistical results with P <0.05 and P <0.01 as a statistically significant difference.