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目的了解江苏省金湖县广州管圆线虫传播宿主分布情况和感染状况,为制定广州管圆线虫病监测方案提供依据。方法选择金湖县前锋镇为纵向监测点,对监测点的野外现场、螺类养殖场、餐饮场所、自由市场等4类场所进行广州管圆线虫终末宿主和中间宿主调查。解剖鼠类心、肺,检查心血管中广州管圆线虫感染情况。采用“肺检查法”结合“酶消化法”或“匀浆法”检测螺类及蛞蝓、蜗牛。利用统一问卷调查当地人群广州管圆线虫病防治知识知晓情况及生食肉情况。结果 2009-2013年调查野外现场15个,餐饮场所21家,螺类养殖场3家,农牧市场9家。捕获褐家鼠11只,黑线姬鼠38只,抓获铜锈环棱螺560个,中华圆田螺101个,蛞蝓494条,蜗牛468个,经解剖和检验未发现广州管圆线虫成虫和幼虫。当地市场上有出售大田螺等现象,居民有吃田螺、青蛙、蟹等习俗。调查目标人群326人,广州管圆线虫病防治知识知晓率为32.82%。学生防治知识知晓率高于当地居民(χ2=17.83,P<0.01)和农牧市场人员(χ2=9.94,P<0.01),差异均有统计学意义。6.44%的人群曾经有生食或半生食鱼、虾史,各人群间生食史率差异无统计学意义(χ2=2.85,P>0.05)。结论金湖县虽为广州管圆线虫病非流行区,但仍应加强宣传教育,注意饮食卫生,以预防广州管圆线虫病的暴发流行。
Objective To understand the host distribution and infection status of C. elegans in Jinhu County, Jiangsu Province, and to provide a basis for the development of the program for monitoring. Methods Qianfeng Town, Jinhu County, was selected as the longitudinal monitoring point to investigate the terminal host and intermediate hosts of C. elegans in 4 sites including field sites, snail farms, food and beverage outlets and free market at the monitoring sites. Anatomy of the mouse heart, lung, check the angiostrongyliasis infection in Guangzhou. Using “lung examination ” combined with “enzyme digestion ” or “homogenization ” detection of snails and catfish, snails. Using the Unified Questionnaire to Survey the Knowledge about the Status of Belonging Cnidaria and the Situation of Raw Edible Meat in Local Populations. Results From 2009 to 2013, there were 15 field sites, 21 dining sites, 3 snail farms and 9 farming and pastoral markets. 11 Rattus norvegicus and 38 Apodemus agrarius were captured, 560 Brassica rapae were collected, 101 RAPD, 494 RAPD and 468 snail were collected. The adults and larvae of C. elegans were not found by anatomy and test. On the local market there is the sale of snails and other phenomena, residents eat snails, frogs, crabs and other customs. The survey target population of 326 people, Guangzhou angiostrong disease prevention knowledge awareness rate was 32.82%. The awareness rate of prevention and treatment of students was higher than that of local residents (χ2 = 17.83, P <0.01) and farmers in the market (χ2 = 9.94, P <0.01). The differences were statistically significant. 6.44% of the population used to have raw or semi-raw fish and shrimp history, and there was no significant difference in the raw food-eating history between the groups (χ2 = 2.85, P> 0.05). Conclusion Although Jinhu County is a non-endemic area of angiostrongyliasis in Guangzhou, publicity and education should still be stepped up to pay attention to food hygiene so as to prevent outbreaks of angiostrongyliasis in Guangzhou.