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90%以上的宫颈癌含有各种致瘤人乳头状瘤病毒(HPV),尤其是HPV16。生殖器HPV感染伴发肿瘤与否可由临床检查发现,包括阴道镜、细胞学、指导性活检的组织学及免疫细胞化学检查。而特异性病毒型只能用DNA杂交技术鉴定,如Southern印迹杂交与Filter原位杂交(FISH)技术。急需一种能筛选那些HPV感染而宫颈癌变危险性较大的妇女的方法,这对防止HPV的进一步扩散也很重要。目前有许多方法用来检测HPV以分析其与宫颈肿瘤的关系。本文分析了非典型巴氏涂片妇女各种HPV-DNA型的分布,并比较现行各种诊断HPV方法的敏感性。病人为初次巴氏涂片异常者,共168例。除详细询问病史外,均行阴道镜检及第二次巴氏涂片。
More than 90% of cervical cancers contain a variety of oncogenic human papillomavirus (HPV), especially HPV16. Genital HPV infection with tumor or not can be found by clinical examination, including colposcopy, cytology, histology and immunocytochemical examination of biopsy. The specific virus type can only be identified by DNA hybridization techniques, such as Southern blot hybridization and Filter in situ hybridization (FISH) technology. There is an urgent need for a method that can screen for women who are at high risk of developing cervical cancer who are HPV-infected, which is also important in preventing the further spread of HPV. There are many ways to detect HPV to analyze its relationship with cervical cancer. This article analyzes the distribution of HPV-DNA types in women who have undergone atypical Pap smear and compares the current sensitivity of various HPV diagnostic methods. Patients with abnormal Pap smear first, a total of 168 cases. Except for detailed history, colposcopy and second Pap smear were performed.