目的研究８ＢｒｃＡＭＰ对培养的人视网膜母细胞瘤ＨＸＯＲｂ４４细胞癌基因表达的效应及其与该细胞生长的关系。方法ｃｆｏｓｍＲＮＡ、ＮｍｙｃｍＲＮＡ及ｐ２１ｒａｓｍＲＮＡ均以原位杂交和ＲＮＡ斑点印迹技术检测，对繁殖细胞核抗原（ＰＣＮＡ）、ｃＦｏｓ、ＮＭｙｃ和Ｐ２１ｒａｓ蛋白表达的免疫反应性（ＩＲ）则采用免疫组化及蛋白质斑点印迹技术检测。结果在人ＨＸＯＲｂ４４细胞，ｃｆｏｓｍＲＮＡ、ＮｍｙｃｍＲＮＡ及ｐ２１ｒａｓｍＲＮＡ定位于胞质，ｃＦｏｓＩＲ及ＮＭｙｃＩＲ定位于胞核，而Ｐ２１ｒａｓＩＲ则位于胞质。斑点印迹的相对扫描数值显示实验组（８ＢｒｃＡＭＰ处理）均低于相应各对照组（未经８ＢｒｃＡＭＰ处理），Ｐ＜０．０５～０．０１。结论（１）８ＢｒｃＡＭＰ可抑制人ＨＸＯＲｂ４４细胞的生长增殖；（２）８ＢｒｃＡＭＰ可下调ｃｆｏｓ、Ｎｍｙｃ及ｐ２１ｒａｓ癌基因的表达；（３）８ＢｒｃＡＭＰ也可下调ｃＦｏｓ、ＮＭｙｃ及Ｐ２１ｒａｓ蛋白的表达，而且其变动各与其ｍＲＮＡ基因表达的变动相一致；（４）结果提示８ＢｒｃＡＭＰ可能从不同的途径而协同抑制ＨＸＯＲｂ４４细胞的生长增殖。 Objective To study the effect of 8BrcAMP on oncogene expression in cultured human retinoblastoma HXORb44 cells and its relationship with the cell growth. Methods c  fos mRNA, N  mycm RNA and p21rasmRNA were detected by in situ hybridization and RNA dot blot technique. The immunoreactivity (IR) to the expression of proliferating cell nuclear antigen (PCNA), cFos, NMyc and P21ras protein Immunohistochemistry and protein blotting detection. Results In human HXORb44 cells, cfosmRNA, NmycmRNA and p21rasmRNA were located in the cytoplasm, cFosIR and NMycIR were located in the nucleus, whereas P21rasIR was located in the cytoplasm. Dot blot relative scanning values ​​show that the experimental group (8  BrcAMP treatment) were lower than the corresponding control group (without 8  BrcAMP treatment), P <0.05 ~ 0.01. (2) 8BrcAMP down-regulated cfos, Nmyc and p21ras oncogene expression; (3) 8BrcAMP can inhibit the growth and proliferation of human HXORb44 cells; CAMP also down-regulated c-Fos, N-Myc and P21ras protein expression, and its changes were consistent with the changes of its mRNA gene expression; (4) The results suggest that 8  BrcAMP may synergistically inhibit HXO from different ways  Rb44 cell growth and proliferation.