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耳蜗标本的几何形态复杂,在组织结构方面又有质量密度差异很大的骨迷路和膜迷路,制备扫描标本时很易造成人工损伤,难以得到理想的标本。现将我们在制备耳蜗扫描标本中的初步体会介绍如下。 1.用灌注法与浸润法联合固定。由于耳蜗结构有大量的骨质,上皮直接贴附于骨质上,两种成份质量密度差异很大,故容易分离断裂,而毛细胞的听毛很细弱,极易损伤和自溶。因此固定很重要,
Cochlear specimens of the complex geometry, in the organizational structure has a large difference in mass density of bone lost and labyrinth of the membrane, the preparation of the specimen is easy to cause artificial damage, it is difficult to get the ideal specimen. Now we are in the preparation of cochlear scan specimens in the initial experience described below. 1 perfusion method and infiltration method combined fixed. Because the cochlear structure has a lot of bone, the epithelium directly attached to the bone, the two components of the mass density difference is very large, it is easy to break apart, while hair cells listen to the hair is very weak, easily damaged and autolysis. So fixed is very important,