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以新疆大叶紫花苜蓿为试验材料,采用土培试验方法,研究了硒(Se)胁迫对大叶紫花苜蓿不同组织可溶性蛋白、脯氨酸、超氧阴离子和丙二醛(MDA)含量的影响及大叶紫花苜蓿对Se的积累能力。结果表明:胁迫60d时,随Se胁迫浓度的增加,大叶紫花苜蓿不同组织中超氧阴离子、MDA、脯氨酸和可溶性蛋白质含量增加,同时,新疆大叶紫花苜蓿根的Se积累量大于叶和茎的Se积累量。综合研究表明,在900μM Se胁迫下,根据渗透调节物质的积累量,初步判断大叶紫花苜蓿有解毒和富集Se的能力,具有用于修复Se污染土壤的潜能。
In this paper, Xinjiang Alfalfa (Medicago sativa) as test material, the effects of Se stress on the contents of soluble protein, proline, superoxide anion and malondialdehyde (MDA) in different tissues of Alfalfa And the capacity of large-leaf alfalfa to accumulate Se. The results showed that the content of superoxide anion, malondialdehyde, proline and soluble protein increased in different tissues of the large-leaf alfalfa with the increase of Se stress at the 60th day. At the same time, the accumulation of Se in Xinjiang’s big-leaf alfalfa was greater than that of leaves and Se accumulation of stems. Comprehensive studies showed that under the stress of 900μM Se, the capacity of alfalfa to detoxify and enrich Se was first determined based on the accumulation of osmotic adjusting substances, which has the potential to repair Se contaminated soil.