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目的探讨子宫内膜癌细胞Ishikawa和Hec-1A分别经胰岛素、力朴素以及胰岛素联合力朴素作用后,对其抑制、凋亡、周期及凋亡抑制基因survivin表达的影响。方法根据已采用的MTT方法确定100 pmol/L胰岛素和1μmol/L力朴素为有效实验浓度。应用流式检测学方法检测各实验组不同时间(24 h、48 h、72 h)Ishikawa和Hec-1A细胞凋亡及周期情况;采用逆转录聚合酶链反应(RT-PCR)检测各实验组24 h时Ishikawa和Hec-1A细胞中凋亡抑制因子Survivin的表达及其差异。结果胰岛素联合力朴素组Ishikawa及Hec-1A细胞的凋亡率随着不同时间点凋亡率持续地增加(P<0.05);联合作用组抑制率明显多于力朴素单独作用组(P<0.05);联合作用Ishikawa细胞时,其周期主要阻滞于G1期;联合作用Hec-1A细胞时,其周期主要阻滞于G1期和G2/M期;联合作用于Ishikawa细胞后,与力朴素组单独作用相比,survivin表达差异无统计学意义(P>0.05);联合作用于Hec-1A细胞后,联合作用组survivin的表达明显多于力朴素组(P<0.05)。结论联合作用组比力朴素单独作用组明显抑制体外Ishikawa和Hec-1A细胞增殖,且两种药物联合作用,可明显下调Survivin在Hec-1A细胞中的表达。
Objective To investigate the effect of Ishikawa and Hec-1A on the expression of apoptosis-suppressor, survivin, apoptosis, cycle and apoptosis inhibitor Ishikawa and Hec-1A after insulin and Natriuretic Peptide combined with Naive Force. Methods Based on the established MTT method, 100 pmol / L insulin and 1 μmol / L force naive were tested as valid experimental concentrations. Flow cytometry was used to detect the apoptosis and cycle of Ishikawa and Hec-1A cells at different time points (24 h, 48 h, 72 h) in each experimental group. Reverse transcription-polymerase chain reaction (RT-PCR) The expression of Survivin and its difference in Ishikawa and Hec-1A cells at 24 h. Results The apoptotic rates of Ishikawa and Hec-1A cells in the combined insulin natriuretic group continued to increase with the apoptosis rate at different time points (P <0.05), and the inhibition rates in the combination group were significantly higher than those in the natriuretic alone group (P <0.05) ), When combined with Ishikawa cells, the cell cycle was mainly arrested in G1 phase. When combined with Hesh-1 cells, the cycle arrest was mainly in G1 phase and G2 / M phase. When combined with Ishikawa cells, There was no significant difference in survivin expression between the two groups (P> 0.05). After combined treatment with Hec-1A cells, the expression of survivin in combination group was more than that in the control group (P <0.05). Conclusions Compared with the naive group, the inhibitory effects on the proliferation of Ishikawa and Hec-1A cells in vitro were significantly inhibited by the combination of two drugs, which could significantly down-regulate the expression of Survivin in Hec-1A cells.