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目的 观察急性肺损伤 (ALI)肺组织核因子 (NF) -κB、肿瘤坏死因子 (TNF) -α的活性变化及地塞米松 (Dex)的干预作用。方法 采用ALI大鼠模型 ,实验动物随机分为ALI模型组 (8只 )、Dex治疗组 (8只 )、正常对照组 (8只 ) ,用免疫组织化学法结合原位定量分析检测肺组织NF -κB、IκB -a、TNF -α蛋白表达及相对含量 ,并进行肺组织的病理学光镜检查。结果 ALI大鼠肺组织NF -κB、TNF -α的蛋白表达明显升高 ,I-κB表达显著降低。Dex能明显下调NF -κB、TNF -α的蛋白表达 ,上调I-κB表达 ,并能减轻肺组织的损伤程度。结论 NF -κB活化在ALI的发生、发展过程中起着重要作用。Dex发挥抗炎作用 ,减少肺组织损害 ,与其对NF -κB的活性和细胞因子的调节作用密切相关。
Objective To observe the changes of activity of nuclear factor (NF) -κB and tumor necrosis factor (TNF) -α in lung tissue of acute lung injury (ALI) and the effect of dexamethasone (Dex). Methods ALI rats were randomly divided into four groups: ALI model group (n = 8), Dex treatment group (n = 8) and normal control group (n = 8). Immunohistochemistry and in situ quantitative analysis were used to detect NF -κB, IκB-a, TNF-α protein expression and relative content, and lung tissue pathology light microscopy. Results The protein expressions of NF-κB and TNF-α in lung tissue of ALI rats were significantly increased, and the expression of I-κB was significantly decreased. Dex can significantly down-regulate the expression of NF-κB and TNF-α, up-regulate the expression of I-κB and alleviate the damage of lung tissue. Conclusion NF-κB activation plays an important role in the development of ALI. Dex exerts anti-inflammatory effects and reduces lung tissue damage, which is closely related to the regulation of NF-κB activity and cytokines.