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目的:研究饿蚂蝗总黄酮对小鼠免疫性肝损伤的保护作用及其机制。方法:72只小鼠随机分为正常对照组、模型组、联苯双酯组(200mg/kg)、饿蚂蝗总黄酮组(800,400,200mg/kg),各组预防性灌胃给药10 d。第10 d,除正常对照组外,其余各组小鼠尾静脉注射刀豆蛋白A(Con A)20 mg/kg诱导免疫性肝损伤,8 h后测定小鼠肝、脾、胸腺指数,检测小鼠肝匀浆中谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量变化,流式细胞术测定外周血中CD4~+、CD8~+T细胞亚群比率,酶联免疫吸附试验(ELISA)测定血清白介素-2(IL-2)、γ干扰素(IFN-γ)、白介素-4(IL-4)、白介素-10(IL-10)的水平。结果:与模型组比较,饿蚂蝗总黄酮(800、400、200 mg/kg)能明显降低肝脏指数,降低肝匀浆中ALT、AST、MDA含量和升高SOD活性,提高外周血CD4~+比率;饿蚂蝗总黄酮(800 mg/kg)能提高外周血CD8~+比率;饿蚂蝗总黄酮(800,400 mg/kg)明显降低脾脏指数,增大胸腺指数,显著降低血清中IL-2、IFN-γ、IL-10水平;饿蚂蝗总黄酮(200 mg/kg)能显著降低血清中IL-2、IL-10水平。结论:饿蚂蝗总黄酮对Con A诱导的免疫性肝损伤有保护作用,其机制与其抗炎、抗氧化以及免疫调节有关。
Objective: To study the protective effect and mechanism of total flavonoids from Locusta sinensis on immunological liver injury in mice. Methods: 72 mice were randomly divided into normal control group, model group, bifendate group (200mg / kg), hungry bitter total flavonoids group (800,400,200mg / kg) . On the 10th day, except for the normal control group, mice in each group were injected with Con A (20 mg / kg) to induce immune liver injury, and the indexes of liver, spleen and thymus were measured 8 h later, The levels of ALT, AST, SOD and MDA in the liver homogenates of mice were measured by flow cytometry. The levels of CD4 ~ +, CD8 ~ + T cell subsets and the levels of IL-2, IFN-γ, IL-4 and IL-10 in serum were measured by enzyme linked immunosorbent assay (ELISA) -10) level. Results: Compared with the model group, total Flavone of hungry grasshopper (800,400,200 mg / kg) could significantly reduce the liver index, reduce the content of ALT, AST and MDA in liver homogenate and increase the activity of SOD, (800 mg / kg) could increase the ratio of CD8 + in peripheral blood. The total flavonoids of 800 mg / kg (800, 400 mg / kg) significantly decreased the spleen index, increased the thymus index, and significantly decreased the serum levels of IL- 2, IFN-γ and IL-10 levels. Serum total IL-2 and IL-10 levels of 200 mg / kg were significantly lower than those of the other two groups. CONCLUSION: The total flavonoids from the hungry locust have a protective effect on immune-induced liver injury induced by Con A, and its mechanism is related to its anti-inflammatory, anti-oxidation and immune regulation.