论文部分内容阅读
建立了一种检测人尿中神经鞘氨醇（So）和二氢神经鞘氨醇（Sa）的高效液相色谱方法（HPLC）。离心分离尿样中的片状剥落细胞，裂解后用乙酸乙酯萃取、邻苯二甲醛衍生，在HPLC系统中通过梯度洗脱用Nova-PakC18－RP色谱柱（15cm×3．9mm，4μm）分离、荧光检测器检测。So和Sa的检出限均为0．05ng（女性尿样0，075μg/L、男性尿样0．005μg/L）。分析从我国一个村在采集的40份尿样，女性尿样中So、Sa和Sa／So比值分别为1．29－13．58μg/L、0．25－3．13μg/L和0．15－0．25，男性尿样中分别为0．075~3．07μg/L、0.019－0．50μg/L和0．028～0．26。
A high performance liquid chromatography (HPLC) method was developed for the determination of sphingosine (sphingosine) and dihydrosphingosine (Sa) in human urine. The exfoliated flakes in the urine samples were centrifuged, lysed, extracted with ethyl acetate, derivatized with o-phthaldialdehyde and purified by Nova-Pak C18-RP column (15 cm x 3.9 mm, 4 μm) Separation, fluorescence detector detection. The detection limits of So and Sa were 0.05ng (0,075μg / L for female urine and 0.005μg / L for male urine). The ratios of So, Sa and Sa / So in the urine samples from 40 urine samples collected from one village in China were 1.29-13.58μg / L, 0.25-3.13μg / L and 0.15 -0.25, respectively. The male urine samples were 0.075-3.07 μg / L, 0.019-0.50 μg / L and 0.028-0.26, respectively.