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提高肿瘤浸润淋巴细胞（ＴＩＬ）的增殖能力和杀伤活性，是其临床应用前需解决的关键问题。为此，作者对ＴＩＬ的分离和培养条件进行了探讨，建立了以机械分散法，０．０５％胶原酶和０．００３％ＤＮＡ酶室温消化６小时加冷消化１８小时的酶消化法和７５％与１００％Ｆｉｃｏｌｌ非连续密度梯度离心法相结合的分离纯化ＴＩＬ方法。分离出的ＴＩＬ在体外条件培养基中多数能扩增到１０９以上的应用标准，ＮＫ、ＬＡＫ活性分别可达５８．３±１１．７％和４８．６±１０．６％，其中发挥主要作用的是ＣＤ８Ｔ细胞。因此，本法不失为一种有效的分离培养ＴＩＬ的方法。它的建立为应用ＴＩＬ治疗恶性肿瘤奠定了良好的实验基础。
To improve the proliferation and cytotoxicity of tumor-infiltrating lymphocytes (TILs) is a key problem to be solved before clinical application. To this end, the author of TIL separation and culture conditions were discussed, the establishment of a mechanical dispersion method, the establishment of 0.05% collagenase and 0.003% DNase digestion at room temperature for 6 hours plus cold digestion 18 hours of enzyme digestion and 75 % Purification of TIL with 100% Ficoll Discontinuous Density Gradient Centrifugation. The isolated TILs could be expanded to more than 109 in the in vitro culture medium, and the activities of NK and LAK reached 58.3 ± 11.7% and 48.6 ± 10.6% respectively, which play a major role Is CD8T cells. Therefore, this method is an effective way to isolate and cultivate TIL. Its establishment has laid a good experimental foundation for the application of TIL in the treatment of malignant tumors.