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以具有产业化前景的微拟球藻Nannochloropsis oceanica OZ-1为实验材料,利用碳重离子进行诱变育种,采用Imaging-PAM和酶标仪进行大规模筛选,最终获得两株高生长速率微拟球藻突变藻株(HP-1和HP-2),进一步分析显示两株突变藻株(HP-1和HP-2)生物量积累较野生型藻株大幅提高,在18 d培养末期生物量分别提高了18%和26%,两株突变藻株油脂产率分别为295 mg/(L.d)和275 mg/(L.d),而野生型藻株为247 mg/(L.d)。所获两株突变藻株生长速度快、油脂产率高,较野生型藻株优势明显。
Nannochloropsis oceanica OZ-1, an industrialized prospect, was used as the experimental material. Mutation breeding was carried out by using carbon-heavy ion and large-scale screening was carried out by using Imaging-PAM and microplate reader. Finally, two strains with high growth rate Further analysis showed that the biomass accumulation of two strains of mutant algae (HP-1 and HP-2) increased significantly compared with that of wild-type algae. After the biomass of 18 d Increased by 18% and 26% respectively. The lipid yield of the two mutant strains was 295 mg / (Ld) and 275 mg / (Ld), respectively, while that of the wild type strain was 247 mg / (Ld). The two mutant strains obtained grew fast and their oil yield was higher than that of wild-type algae.