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目的:研究巴曲酶对沙土鼠前脑缺血后行为学和组织病理学的影响。方法:采用沙土鼠前脑缺血模型,缺血时间10min。动物随机分为3组:假手术组、常温再灌注组、巴曲酶再灌注组,(n=7)。巴曲酶8B~U·kg~(-1)在再灌注30min经腹腔注入。动物存活第5天时行开阔法行为学检查,第7天时行海马CA1区组织病理学检查。结果:开阔法行为学检查显示,常温再灌注组沙土鼠的探索活动较假手术组活跃(P＜0.01)。巴曲酶组沙土鼠的探索活动较常温再灌注组弱(P＜0.05),但较假手术组活跃(P＜0.05)。组织病理学检查显示,巴曲酶组海马CA1区内侧、中间和外则存活神经元计数较常温再灌注组多620%、470%和200%(P均＜0.01),较假手术组少64%(P＜0.01)、43%(P＜0.05)和30%。结论:沙土鼠前脑缺血后应用巴曲酶治疗,能够减轻动物神经功能障碍,减少海马CA1神经坏死。
Objective: To study the effect of batroxobin on the behavior and histopathology of gerbils after forebrain ischemia. Methods: The gerbil forebrain ischemia model was used. The ischemic time was 10 min. Animals were randomly divided into 3 groups: sham operation group, normal temperature reperfusion group, batroxobin reperfusion group, (n = 7). Batroxobin 8B ~ U · kg ~ (-1) was injected intraperitoneally 30 min after reperfusion. Animals were sacrificed on day 5 for behavioral examination. Hippocampal CA1 region histopathology was performed on the 7th day. Results: The open method behavioral examination revealed that the gerbils in the normal temperature reperfusion group were more active than the sham group (P <0.01). The activities of gerbils in Batroxobin group were weaker than those in normal temperature reperfusion group (P <0.05), but were more active than sham group (P <0.05). Histopathological examination showed that the neurons in the medial, middle and outside of CA1 area of Batroxobin group were 620%, 470% and 200% more than normal temperature reperfusion group (P <0.01), 64 % (P <0.01), 43% (P <0.05), and 30%. Conclusion: The use of batroxobin after forebrain ischemia in gerbils can reduce neurological dysfunction and reduce neuronal necrosis of hippocampal CA1.