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目的通过检测血清样本中HBVDNA、乙型肝炎病毒大蛋白(LHBs)、乙型肝炎五项指标以及乙型肝炎前S1(PreS1Ag),探讨LHBs用于乙型肝炎临床诊断的价值及与其他乙型肝炎病毒标志的相关性。方法收集623份乙型肝炎患者血清标本,LHBs、乙型肝炎五项指标以及PreS1Ag,采用ELISA技术检测了HBsAg阳性血清标本中的LHBs、HbeAg、PreS1Ag抗原,采用荧光定量PCR技术检测HBVDNA。结果 515份HBVDNA阳性血清标本中,LHBs检测阳性485份(94.17%);PreS1Ag检测阳性260份(50.49%);LHBs阳性率明显高于PreS1Ag阳性率,两者差异有统计学意义(P<0.01);在检测270份HBeAg阴性血清标本中,HBVDNA检出阳性133份(49.26%),LHBs检出阳性119份(44.07%),两者差异无统计学意义;LHBs吸光度(OD值)与HBVDNA呈正相关关系(r=0.980)。结论 LHBs可用于反映乙型肝炎病毒复制水平的敏感新指标,操作方法便捷适合临床应用。
Objective To investigate the value of LHBs in the clinical diagnosis of Hepatitis B by detecting HBVDNA, LHBs, B hepatitis B and PreS1Ag in serum samples, Correlation of hepatitis virus markers. Methods The serum samples, LHBs, hepatitis B and PreS1Ag of 623 hepatitis B patients were collected. The LHBs, HbeAg and PreS1Ag antigens in HBsAg positive serum samples were detected by ELISA. HBVDNA was detected by fluorescence quantitative PCR. Results The positive rate of LHBs was 485 (94.17%) in 515 HBVDNA positive sera samples, 260 (50.49%) were positive for PreS1Ag, the positive rate of LHBs was significantly higher than that of PreS1Ag, the difference was statistically significant (P <0.01) ). Of the 270 samples with HBeAg negative serum, 133 (49.26%) positive for HBVDNA and 119 (44.07%) for LHBs were positive, and there was no significant difference between the two. There was no significant difference between LHBs absorbance (OD) and HBVDNA There was a positive correlation (r = 0.980). Conclusion LHBs can be used to reflect the new level of hepatitis B virus replication sensitive indicators, convenient and suitable for clinical practice.