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通过分析海绵清除大肠杆菌的过程,研究海绵净化细菌的机理。作者利用荧光显微镜和激光共聚焦显微镜观测等手段,监测和分析了绿色荧光大肠杆菌(Escherichia coli)在繁茂膜海绵(Hymeniacidon perlevis)体内、体外水环境中数量变化过程。在1 L含有3×107个/m L绿色荧光大肠杆菌的海水中放入鲜重(1.02±0.11)g的繁茂膜海绵24块,处理7 h,海水中的荧光大肠杆菌数量逐渐降低;而海绵体内荧光大肠杆菌数量在2 h时内逐渐增多,之后的2 h趋于稳定,4 h以后开始逐渐减少。水体中大肠杆菌不仅进入海绵体内,而且进入海绵细胞内。含有荧光大肠杆菌的海绵块转入无菌海水中后,海绵体内及细胞中大肠杆菌逐渐消失,而且大肠杆菌没有被释放到环境海水中。分析表明,繁茂膜海绵能够以摄食的方式净化水环境中的大肠杆菌。
By analyzing the process of sponge removal Escherichia coli, sponge bacteria to study the mechanism of cleaning. Using fluorescence microscopy and confocal laser scanning microscopy, the authors monitored and analyzed the quantitative changes of green fluorescent Escherichia coli in the in vitro and in vivo water environment of the hymeniacidon perlevis. In fresh seawater containing 3 × 107 cells / m L of green fluorescent E.coli, 1 L of fresh lumps of sponge membrane (1.02 ± 0.11 g) was immersed in 1 L for 7 h, and the number of fluorescent E. coli in seawater gradually decreased. The number of Escherichia coli in sponge increased gradually within 2 h, then stabilized at 2 h, and gradually decreased after 4 h. E. coli in water not only enter the sponge, but also enter the sponge cells. After the sponge containing fluorescent Escherichia coli is transferred into sterile seawater, Escherichia coli in the sponge body and cells gradually disappear, and Escherichia coli is not released into the environment seawater. The analysis shows that lush membrane sponges are able to purify Escherichia coli in the water environment by ingestion.