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目的:研究四逆散改善失眠作用的机制。方法:四逆散浸膏按21.7 g.kg-1.d-1给SD大鼠连续灌胃7 d,腹主动脉取血,分离血清,按10%比例加进原代培养SD大鼠皮层神经元培养基,37℃孵育3 h,应用全细胞膜片钳方法,记录四逆散含药血清对原代培养大鼠皮层神经元γ-氨基丁酸A型受体(GABAA)介导的氯离子(Cl-)电流的影响。结果:给予0.3,3,30,300μmol.L-1γ-氨基丁酸刺激,经10%四逆散含药血清作用3 h的皮层神经元,γ-氨基丁酸浓度-效应曲线左移,半效激活浓度降低(P=0.05),氯离子电流的最大峰值(P<0.05)、快速衰减时间常数(τ2,P<0.05)和慢速衰减时间常数(τ1,P<0.05)明显增大。结论:四逆散作用于皮层神经元的γ-氨基丁酸A型受体,调节其介导的氯离子电流,可能是其改善失眠作用的机制之一。
Objective: To study the mechanism of Sini inverse in improving insomnia. Methods: Sini San extract at 21.7 g.kg-1.d-1 SD rats were fed for 7 days, the abdominal aorta blood, serum was separated by 10% added to primary cultured SD rat cortex Neuron culture medium and incubate at 37 ℃ for 3 h. Whole-cell patch clamp method was used to record the effect of Sini-San-containing serum on the proliferation of primary cultured rat cortical neurons induced by γ-aminobutyric acid type A receptor (GABAA) Ion (Cl-) current effects. RESULTS: After stimulation with 0.3, 3, 30, 300μmol.L-1γ-aminobutyric acid for 3 h, the γ-aminobutyric acid concentration-effect curve shifted to the left, half-effect (P <0.05), fast decay time constant (τ2, P <0.05) and slow decay time constant (τ1, P <0.05) increased significantly. CONCLUSIONS: Sini Powder acts on γ-aminobutyric acid type A receptors in cortical neurons and regulates its mediated chloride current, which may be one of the mechanisms of its effect on improving insomnia.