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目的:通过检测不同程度颅脑外伤患者血浆中一氧化氮(NO)、内皮素(ET)和白细胞介素-6(IL-6)水平,探讨其在颅脑外伤中的临床意义。方法:选择本院102例颅脑外伤的患者作为病例组,并按照GCS评分将其分为重度组(37例)、中度组(34例)和轻度组(31例)三组,选取同期在我院健康体检者38例作为对照组。采集所有研究对象静脉血,分别采用固相夹心ELISA法、放射免疫分析法和ELISA检测其血浆中的NO、ET和IL-6含量。结果:不同程度颅脑外伤患者血液中NO、ET和IL-6含量和对照组比较总体差异均有统计学意义(P<0.01),Dunnettt检验发现病例组三个程度组中NO、ET和IL-6与对照组中相比差异均具有统计学意义(P<0.01)。NO和ET在四组间含量依次是颅脑外伤重度组>颅脑外伤中度组>颅脑外伤轻度组>健康对照组;IL-6在四组中含量从高到低依次是颅脑外伤重度组>颅脑外伤中度组>健康对照组>颅脑外伤轻度组(P<0.01)。Sperm an秩相关分析,发现NO、ET、IL-6含量均与病情程度呈正相关(rNO=0.907,rET=0.887,rIL-6=0.784,P<0.01)。结论:不同程度颅脑外伤患者血浆中NO、ET和IL-6水平的检测对颅脑外伤者的预后情况判断具有一定的临床意义。
Objective: To investigate the clinical significance of plasma levels of nitric oxide (NO), endothelin (ET) and interleukin-6 (IL-6) in patients with craniocerebral trauma with different degrees of traumatic brain injury. Methods: A total of 102 patients with craniocerebral trauma were selected as the case group and divided into three groups: severe group (37 cases), moderate group (34 cases) and mild group (31 cases) according to GCS score. The same period in our hospital 38 healthy subjects as a control group. Venous blood was collected from all the subjects. The levels of NO, ET and IL-6 in plasma were detected by solid phase sandwich ELISA, radioimmunoassay and ELISA respectively. Results: The levels of NO, ET and IL-6 in the blood of patients with craniocerebral trauma were significantly different from those in the control group (P <0.01). The levels of NO, ET and IL in the three groups -6 compared with the control group, the difference was statistically significant (P <0.01). The contents of NO and ET in the four groups were as follows: severe traumatic brain injury> moderate traumatic brain injury> mild traumatic brain injury> healthy control group. The content of IL-6 in high, Severe trauma group> moderate traumatic brain injury group> healthy control group> mild traumatic brain injury group (P <0.01). Sperm an rank correlation analysis found that NO, ET, IL-6 levels were positively correlated with the severity of the disease (rNO = 0.907, rET = 0.887, rIL-6 = 0.784, P <0.01). Conclusion: The levels of NO, ET and IL-6 in patients with craniocerebral trauma have different clinical significance in judging the prognosis of patients with craniocerebral trauma.