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应用放射配体结合测定法，研究老年鼠睾丸糖皮质激素受体（ＧＲ）的变化。结果表明，老年鼠睾丸细胞液ＧＲ的最大结合容量（Ｒｏ）为４８．４３±１１．８７ｆｍｏｌ／ｍｇ蛋白，显著低于成年鼠的５９．６９±１２．７３ｆｍｏｌ／ｍｇ蛋白（Ｐ＜０．０１），ＧＲ的平衡解离常数（Ｋｄ）无显著性变化（Ｐ＞０．０５）。同时测定了血浆皮质醇、睾酮浓度及外周血白细胞ＧＲ的特异结合量（Ｒｓ）和睾丸细胞液磷脂酶Ａ２的含量，探讨了ＧＲ变化与衰老发生的关系。
Radioligand binding assay was used to study the changes of testicular glucocorticoid receptor (GR) in aged rats. The results showed that the maximum binding capacity (GR) of GR in testicular cells of aged mice was 48.43 ± 11.87 fmol / mg protein, which was significantly lower than 59.69 ± 12.73 fmol / mg protein in adult mice (P <0.01) ), There was no significant change in the equilibrium dissociation constant (Kd) of GR (P> 0.05). The levels of plasma Cortisol, testosterone and specific binding of GR in peripheral leukocytes (Rs) and phospholipase A2 in testis were also determined. The relationship between GR changes and senescence was also discussed.