论文部分内容阅读
目的建立复方盐肤木化痔胶囊中没食子酸含量的测定方法。方法采用HPLC法测定没食子酸的含量。AnglentTC-18柱(4.6×250 mm,5μm),甲醇-0.5%冰醋酸(5∶95)为流动相,柱温为室温,流速为1.0 mL.min-1,检测波长为273 nm。结果没食子酸浓度在15.09～45.27μg.mL-1范围内与其峰面积呈良好的线性关系(r=0.9996,n=7),平均回收率为101.83%(RSD=1.67%)。结论该测定方法精密度高,重现性好,可用于复方盐肤木化痔胶囊的质量控制。
Objective To establish a method for the determination of gallic acid content in Compound Yanmumuhuayu capsules. Methods The content of gallic acid was determined by HPLC. Anglent TC-18 column (4.6×250 mm, 5 μm), methanol-0.5% glacial acetic acid (5:95) was used as the mobile phase, the column temperature was room temperature, the flow rate was 1.0 mL.min-1, and the detection wavelength was 273 nm. Results The concentration of gallic acid in the range of 15.09-45.27μg.mL-1 showed a good linear relationship with its peak area (r=0.9996, n=7). The average recovery was 101.83% (RSD=1.67%). Conclusion The method has high precision and good reproducibility and can be used for quality control of compound Yanfumuhuayu Capsule.