为了研究神经节苷脂ＧＭ３（Ｇａｎｇｌｉｏｓｉｄｅ，ＧＭ３）对人早幼粒白血病细胞系ＨＬ６０细胞诱导分化后中性糖脂的影响，将神经节苷脂ＧＭ３加到ＨＬ６０细胞的ＤＭＥ／Ｆ１２培养液中，观察其对ＨＬ６０细胞的诱导分化作用及分化后细胞中性糖脂的变化。结果：细胞吞噬功能显著增强，非特异性酯酶活性升高，细胞形态也呈现与这种分化相一致的改变。神经节苷脂ＧＭ３诱导ＨＬ６０细胞分化后细胞中性糖脂各组分的百分含量发生了显著改变，ＣＤＨ、ＣＭＨ及ＣＱＨ显著增多，与分化前相比Ｐ＜００５；ＣＸＨ显著降低，与分化前相比Ｐ＜００５。结果提示：神经节苷脂ＧＭ３诱导ＨＬ６０细胞分化时改变了细胞中性糖脂的代谢 In order to study the ganglioside GM3 (Ganglioside, GM3) on human promyelocytic leukemia cell line HL 60 cells induced differentiation of neutral glycolipid GM ganglioside GM3 added to DM60 / D12 The culture medium was used to observe the differentiation and differentiation of HL60 cells and the changes of the cells’ neutral glycolipids after differentiation. Results: Phagocytic function was significantly enhanced, non-specific esterase activity increased, cell morphology also showed a change consistent with this differentiation. Ganglioside GM3 induced HL 60 cell differentiation of cells after the content of cell-free glycolipid percentage had a significant change, CDH, CMH and CQH significantly increased, compared with before differentiation P <0  05; CXC significant Decreased, P <005 compared with that before differentiation. The results suggest that: Ganglioside GM3 induced differentiation of HL 60 cells change the metabolism of cell neutral glycolipids