二株莱氏野村菌分别分离自日本的淡剑贪夜蛾和大豆夜蛾 ,将他们接种于斜纹贪夜蛾的幼虫 ,并饲以人工饲料 .共设置 5个分生孢子悬浮液的浓度梯度 ,即 10 7,10 6 ,10 5,10 4 ,10 3孢子 /毫升 .对斜纹贪夜蛾幼虫的毒力试验结果表明 :分离自淡剑贪夜蛾菌株的LC50为 5 38,分离自大豆夜蛾菌株的LC50为 5 71,10 7孢子 /毫升浓度分生孢子悬浮液的致病死亡率分别为 89 5%和 84 2 % Two Rhizoctonia solani were isolated from Japan, Spodoptera frugiperda and Soybean worms, they were inoculated on the Spodoptera frugiperda larvae, and fed with artificial feed .Set a total of five conidia suspension concentration gradient Ie 10 7, 10 6, 10 5, 10 4, 10 3 spores / ml.The virulence test on Larvae of Spodoptera frugiperda showed that the LC50 isolated from S. frugiperda strain was 538 and was isolated from soybean The LC50 of C. litura was 5 71,107 conidia / mL and the mortalities of the conidial suspension were 89 5% and 84 2%