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目的:探讨宫颈病变组织中整合素连接激酶(integrin-link kinase,ILK)、基质金属蛋白酶-9(matrixmetalloproteinases,MMP-9)的表达及其临床意义。方法:免疫组化方法检测2010-09-01-2012-06-30我院门诊及住院的60例宫颈鳞状细胞癌、100例宫颈上皮内瘤变(cervical intraepithelial neopla,CIN)、40例慢性宫颈炎组织中ILK和MMP-9的表达。结果:1)宫颈癌组织中ILK表达率为80.0%(48/60),明显高于正常宫颈黏膜上皮的0%(0/40),χ2=61.54,P<0.001;高于CINⅠ~Ⅱ的30.77%(16/52),χ2=27.27,P<0.001;也高于CINⅢ的45.83%(22/48),χ2=13.65,P<0.001。而CINⅠ~Ⅱ与CINⅢ之间ILK的表达差异无统计学意义,χ2=2.404,P=0.121。宫颈癌组织中MMP-9表达率为88.33%(53/60),明显高于正常宫颈黏膜上皮(0/40),χ2=75.18,P<0.001;高于CINⅠ~Ⅱ的28.85%(15/52),χ2=41.33,P<0.001;但与CINⅢ的79.17%(38/48)相比差异无统计学意义,χ2=1.69,P=0.194。MMP-9在CINⅢ的表达明显高于CINⅠ~Ⅱ,χ2=25.37,P<0.001。ILK表达与临床分级、组织学分级和淋巴结转移有关,P值均<0.05。MMP-9与淋巴结转移有关,P=0.012。ILK和MMP9在宫颈癌组织中表达呈正相关,r=0.429,P<0.05。结论:ILK、MMP-9在宫颈癌组织中均呈过表达,且两者的过表达可能在宫颈癌浸润转移过程中起重要作用。
Objective: To investigate the expression of integrin-link kinase (ILK) and matrixmetalloproteinases-9 (MMP-9) in cervical lesions and its clinical significance. Methods: Immunohistochemistry was used to detect 60 cases of cervical squamous cell carcinoma, 100 cases of cervical intraepithelial neoplasty (CIN) and 40 cases of chronic The expression of ILK and MMP-9 in cervicitis tissues. Results: 1) The expression of ILK in cervical cancer was 80.0% (48/60), which was significantly higher than that in normal cervical epithelium (0/40), χ2 = 61.54, P <0.001, higher than that of CINⅠ ~ Ⅱ 30.77% (16/52), χ2 = 27.27, P <0.001; also higher than 45.83% (22/48) of CINⅢ, χ2 = 13.65, P <0.001. There was no significant difference in ILK expression between CINⅠ ~ Ⅱ and CINⅢ, χ2 = 2.404, P = 0.121. The positive rate of MMP-9 in cervical cancer was 88.33% (53/60), which was significantly higher than that in normal cervical epithelium (0/40), χ2 = 75.18, P <0.001, higher than that in CINⅠ-Ⅱ (28.85% 52), χ2 = 41.33, P <0.001; but there was no significant difference compared with 79.17% (38/48) of CINⅢ, χ2 = 1.69, P = 0.194. The expression of MMP-9 in CINⅢ was significantly higher than CINⅠ ~ Ⅱ, χ2 = 25.37, P <0.001. ILK expression was associated with clinical grade, histological grade and lymph node metastasis, P <0.05. MMP-9 and lymph node metastasis, P = 0.012. The expression of ILK and MMP9 in cervical cancer was positively correlated, r = 0.429, P <0.05. Conclusion: Both ILK and MMP-9 are overexpressed in cervical cancer, and overexpression of both may play an important role in the invasion and metastasis of cervical cancer.