论文部分内容阅读
目的 通过体外传代培养猪耳廓软骨细胞 ,并连续检测软骨细胞形成外分泌基质的能力 ,寻找出最适宜接种的种子细胞。方法 通过Ⅱ型胶原酶消化法获得高活性的软骨细胞 ,定量接种 6× 10 6个细胞于培养皿 ,每周传代 1次 ,连续 5周 ,留取所有培养液用阿利新蓝法测定软骨细胞外分泌基质糖胺多糖 (GAG) ;利用光镜观察原代及传代软骨细胞的生长情况。结果 第 1,2 ,3 ,4,5周软骨细胞外分泌基质GAG平均含量分别为 :612 ,12 3 5 ,14 62 ,113 8和 710 μg。 结论 体外培养的第 2代软骨细胞功能和活性最佳 ,传代第 2周是最佳接种时间。
Objective To investigate the ability of chondrocytes to form exocrine matrix through subculturing porcine auricle chondrocytes in vitro and find out the most suitable seed cells for inoculation. Methods Highly active chondrocytes were obtained by digestion with type Ⅱ collagenase. 6 × 10 6 cells were inoculated into Petri dishes quantitatively and passaged once a week for 5 weeks. All the culture mediums were taken for determination of cartilage cells Exocrine matrix glycosaminoglycan (GAG); the use of light microscopy observed primary and passaged chondrocytes growth. Results The average GAG content of cartilage extracellular matrix on the 1st, 2nd, 3rd, 4th and 5th weeks were 612, 125, 1462, 113 8 and 710 μg, respectively. Conclusion The second generation of chondrocytes cultured in vitro has the best function and activity, and the second passage of passage is the best time for inoculation.