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品种Lira具有两种生物型,分别称为生物型Υ-42和生物型Υ-45。这两种生物型的分子学分析可用作研究硬粒小麦品种品质高低差异之分子学基础的典型体系,本文即对该体系进行了仔细考查。用来自α-、β-、Υ-醇溶蛋白和高、低分子量麦谷蛋白编码的侧翼核苷酸引物进行生物型Υ-42和生物型Υ-45的聚合酶链反应分析,观测到该两种生物型之间Υ-醇溶蛋白序列的不同扩增谱带。对相同生物型进行的Southern印迹分析表明Υ-醇溶蛋白和低分子量(LMW)麦谷蛋白基因的杂交模式不同,LMW麦谷蛋白序列的杂交模式揭示在生物型Υ-45中存在附加谱带.从而表明生物型Υ-45与生物型Υ-42间决定品质差异的低分子量麦谷蛋白在数量上的差别很可能是由于生物型Υ-45中LMW基因数量高所致。这一结论似乎也得到了属于Υ-42和Υ-45型的其它硬粒小麦品种中所得结果的支持.
The breed Lira has two biotypes, called biotype Y-42 and biotype Y-45, respectively. Molecular analysis of these two biological types can be used as a molecular basis for the study of the differences in the quality of Durum cultivars, a typical system, this article has carried out a careful examination of the system. Polymerase chain reaction analysis of bio-type Y-42 and bio-type Y-45 was performed with flanking nucleotide primers encoded by α-, β-, γ-gliadin and high and low molecular weight glutenins and the two Different amplification bands of the γ-gliadin sequences among the biotypes. Southern blot analysis of the same biotype showed that the hybridization patterns of the γ-gliadin and the low molecular weight (LMW) glutenin genes were different, and the hybridization pattern of the LMW glutenin sequence revealed the presence of an additional band in the biological type Y-45 The quantitative difference between the low molecular weight glutenins indicating the difference in quality between bio-type Y-45 and bio-type Y-42 is most likely due to the high number of LMW genes in bio-type Y-45. This conclusion seems to be supported by the results obtained in other Durum wheat varieties belonging to the Y-42 and Y-45 types.