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目的探讨8羟基脱氧鸟苷(8-hydroxy-2’-deoxyguanosine,8-OHdG)与男性不育精索静脉曲张(varicocele,VC)的相关性.方法选取在本院就诊男性不育VC的41例患者作为实验组,选取具有正常生育力男性33例作为对照组,按照WHO标准测定精液相关参数,利用8-OHdG特异性单克隆抗体试剂盒,应用双抗体夹心酶标免疫分析法测定患者精浆和静脉血中8-OHdG水平;分析8-OhdG与男性不育VC的相关性.结果对照组的精子浓度、前向运动率、存活率、正常形态率均高于实验组,差异具有统计学意义(P<0.05);对照组和实验组的血清8-OHdG水平分别为(44.03±29.23)ng/ml和(62.12±35.52)ng/ml;对照组和实验组的精浆8-OHdG水平分别为(18.68±3.36)ng/ml和(21.20±3.99)ng/ml;实验组精浆和血清8- OHdG水平均高于对照组.精浆8-OHdG与精子存活率、前向运动率呈现负相关关系(r=0.77,P<0.01; r=0.81, P=0.04).血清的8-OHdG水平高于精浆.数据显示患者年龄与精浆8-OhdG水平呈负相关(B=-0.225,P<0.05),年轻患者精浆8-OhdG水平较高.结论男性不育VC患者存在过氧化损伤,过量活性氧产生可能是患者精子质量低下的原因之一.VC与8-OHdG之间存在相关性,8-OHdG可作为一项过氧化检测指标,为临床男性不育诊疗提供有效的参考依据.“,”Objective To study the relationship of 8-hydroxy-2 ’-deoxyguanosine(8-OHdG) and male infertility with varicoceles(VC). Methods Total of 41 male infertility with varicoceles(the experiment group)and 33 well-bearing man(the control group) were enrolled in the study. The levels of 8-OHdG in blood and seminal plasma were detected by EIA. The relationship between 8-OHdG and VC was further explored. Results Sperm concentration, forward motility rate, survival rate and normal morphology of the control group were significantly higher than those of the experiment group. Blood 8-OHdG levels were (44.03±29.23) ng/ml and (62.12±35.52) ng/ml respectively (P<0.05). seminal plasma 8-OHdG levels were (18.68±3.36)ng/ml, (21.20±3.99)ng/ml respectively (P<0.05). Blood 8-OHdG levels were higher than seminal plasma 8-OHdG levels. 8-OHdG levels were negatively related to activity rates and survival rates(r=0.77, P<0.01;r=0.81, P=0.04) . 8-OHdG levels in seminal plasma of young man were higher than that of elders. Conclusion Peroxidative damages might be one reason of poor sperm quality in VC patients. 8-OHdG might be an indication for peroxidize reaction.