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探讨以白细胞介素２（ＩＬ－２）基因修饰的肿瘤细胞作为瘤苗治疗膀胱癌的应用价值。采用脂质体介导法将携带ＩＬ－２基因的逆转录病毒载体转染入膀胱癌细胞系ＢＴＴ７３９中，流式细胞仪行细胞ＤＮＡ周期分析。动物实验观察放射线灭活后基因瘤苗细胞的抗肿瘤免疫作用。建立能分泌ＩＬ－２的膀胱癌瘤苗ＢＴＴ７３９／ＩＬ－２，ＤＮＡ周期分析表明ＩＬ－２基因的导入及表达对ＢＴＴ７３９细胞的增殖无影响。放射线灭活后ＢＴＴ７３９／ＩＬ－２细胞丧失增殖能力，但仍能维持一定水平的ＩＬ－２分泌活性达２周左右。先用灭活的基因瘤苗免疫小鼠，可诱导免疫保护，３周后接种ＢＴＴ７３９不形成肿瘤；用灭活的基因瘤苗治疗荷瘤小鼠，可使５０％小鼠肿瘤消退并长期存活；对治愈后长期存活的小鼠再次接种高剂量ＢＴＴ７３９细胞，仍无肿瘤形成。说明转染ＩＬ－２基因的膀胱癌瘤苗诱导的抗肿瘤作用对预防肿瘤的发生、抑制和清除微小瘤灶，防止膀胱癌复发具有重要的应用价值。
To investigate the value of using interleukin 2 (IL-2) gene modified tumor cells as tumor vaccine in the treatment of bladder cancer. The retroviral vector carrying IL-2 gene was transfected into BTT739 bladder cancer cell line by liposome-mediated method. Cell cycle analysis was performed by flow cytometry. Animal Experiment Observed Antitumor Immunity of Gene Vaccine Cell after Radiation Inactivation. The establishment of bladder cancer vaccine BTT739 / IL-2 secreting IL-2, DNA cycle analysis showed that IL-2 gene import and expression had no effect on the proliferation of BTT739 cells. BTT739 / IL-2 cells lost their proliferative capacity after radiation inactivation, but they still maintained a certain level of IL-2 secretion for about 2 weeks. Immunization of mice with inactivated gene vaccines first induced immune protection and vaccination with BTT739 did not form tumors after 3 weeks; treatment of tumor-bearing mice with inactivated gene vaccines led to tumor regression and long-term survival in 50% of mice ; The long-term survival of mice after re-vaccination of high doses of BTT739 cells, still no tumor formation. It is indicated that the anti-tumor effect induced by bladder cancer vaccine transfected with IL-2 gene has important application value in preventing the occurrence of tumor, inhibiting and eliminating the tiny tumor and preventing the recurrence of bladder cancer.