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作者用遗传工程方法将白喉毒素(DT)A链无毒肽(DTA)基因与百日咳毒素(PT)S1亚单位的C180肽基因重组。构建分4步完成:首先将DTA基因通过质粒M13制成单链,通过定向诱变技术将其F2基因的第208
The authors genetically engineered the diphtheria toxin (DT) A-chain non-toxic peptide (DTA) gene and the Pertussis Toxin (PT) S1 subunit C180 peptide gene. The construction was completed in 4 steps: firstly, the DTA gene was made into a single strand by the plasmid M13, and the 208