Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated

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Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem(ES)cells and blocks their differentiation into endoderrn.The murine F9 embryonal carcinoma cell line is a well-docu-mented model system for endoderm cell lineage differentiation.Here,we examined the function of Nanog in F9 cellendoderm differentiation.Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses thedifferentiation of primitive endoderm and parietal endoderm in F9 cells,whereas it has no effect on the differentiation ofvisceral endoderm.In contrast,the expression of C-terminal domain-truncated Nanog spontaneously promotes endodermdifferentiation in F9 cells.These data suggest that Nanog is required to sustain the proper undifferentiated status of F9cells,and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietalendoderm differentiation in F9 cells. Nanog is a recently discovered discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem (ES) cells and blocks their differentiation into endoderrn. The murine F9 embryonal carcinoma cell line is a well-docu-mented model system for endoderm cell lineage differentiation. Here, we examined the function of Nanog in F9 cellendoderm differentiation. Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses the differentiation of primitive endoderm and parietal endoderm in F9 cells, but it it no effect on the differentiation of visceral endoderm . In contrast, the expression of C-terminal domain-truncated Nanog spontaneously promotes endoderm differentiation in F9 cells. The data suggest that Nanog is required to sustain the proper undifferentiated status of F9 cells, and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietalendoderm differentiation in F9 cells.
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