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顺铂是一种广泛使用的抗癌药,但具有一定的毒性,如:抑制 DNA、RNA 和蛋白质的合成以及氨基酸的转运,还可与半胱氨酸、谷胱甘肽上的巯基结合抑制许多含巯基的酶。本文观察了小鼠预先用 buthioninesulfoximine(BSO,一种γ-谷氨酰半胱氨酸合成酶抑制剂)以及谷胱甘肽和谷胱甘肽单异丙酯处理后对顺铂毒性的防护作用。实验按以下程序进行:将顺铂溶于生理盐水后10min内给小鼠腹腔注射;在给顺铂前16.5h 和5.5h 皮下注射 BSO(3mmol/kg);这些小鼠在
Cisplatin is a widely used anti-cancer drug, but has some toxicity, such as inhibiting the synthesis of DNA, RNA and protein as well as the amino acid transport, but also inhibits the binding of sulfhydryl to cysteine and glutathione Many thiol-containing enzymes. In this paper, the protective effect of cisplatin on the toxicity of mouse pre-treated with buthioninesulfoximine (BSO, a γ-glutamylcysteine synthetase inhibitor) and glutathione and glutathione monoisopropyl ester . The experiment was carried out according to the following procedure: mice were intraperitoneally injected with cisplatin 10 minutes after they were dissolved in saline; BSO (3 mmol / kg) was injected subcutaneously 16.5 hours and 5.5 hours before cisplatin treatment;