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目的观察丙烯酰胺(AA)对L-02肝胎细胞株细胞周期、细胞凋亡的影响以及对DNA的损伤作用。方法以不同终浓度AA(0.01、0.1、0.5、1.0、2.5、5.0和7.5mmol/L)对L-02细胞进行染毒,12h和24h后分别检测细胞存活率、细胞周期、细胞凋亡和DNA损伤。结果与对照组相比,低剂量(0.01mmol/L和0.1mmol/L)染毒细胞存活率略有升高,中剂量(0.5、1.0和2.5mmol/L)、高剂量(5.0mmol/L和7.5mmol/L)则显著降低(P<0.05);G1期细胞含量随染毒剂量的升高逐渐降低,中、高剂量组(>1.0mmol/)G1期细胞含量显著低于对照组(P<0.05),S期细胞含量则相反;中、高剂量(>2.5mmol/L)染毒组细胞凋亡率显著高于对照组(P<0.05);中、高剂量(>1.0mmol/L)染毒细胞拖尾DNA含量以及OTM值显著升高(P<0.05)。结论AA可引起细胞周期紊乱及细胞凋亡,并且对DNA有较强的损伤作用。
Objective To observe the effect of acrylamide (AA) on the cell cycle and apoptosis of L-02 hepatic fetal cell line and its DNA damage. Methods L-02 cells were exposed to various concentrations of AA (0.01,0.1,0.5,1.0,2.5,5.0 and 7.5mmol / L), and the cell viability, cell cycle, apoptosis and DNA damage. Results Compared with the control group, the survival rate of cells exposed to low dose (0.01 mmol / L and 0.1 mmol / L) increased slightly, with medium dose (0.5, 1.0 and 2.5 mmol / L) And 7.5mmol / L) (P <0.05). The content of cells in G1 phase decreased gradually with the increase of the dose, and the content of G1 phase cells in medium and high dose groups (> 1.0mmol / L) (P <0.05), and the content of S phase cells was opposite. The apoptosis rates of cells in medium and high dose (> 2.5mmol / L) group were significantly higher than those in control group (P <0.05) L) DNA content and the OTM of infected cells were significantly increased (P <0.05). Conclusion AA can cause cell cycle disorders and apoptosis, and have a strong DNA damage.