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目的：建立一种可同时分泌多巴胺（DA）和GDNF的工程细胞，并研究其在帕金森病（PD）基因治疗中的可能作用。方法：克隆携带Kozak序列的GDNFcDNA，转染至可分泌DA的MN9D细胞系，将此工程细胞和大鼠原代多巴胶能神经元共培养，免疫组化检测DA能神经元。结果：发现该工程细胞可防止DA能神经元退变死亡，并有助于DA能神经元抵抗MPP+的毒性损伤。结论：本文首次将PD防治兼顾策略结合起来构建MN9D工程细胞，结果表明其在PD的基因治疗中可能具有重要的应用价值。
OBJECTIVE: To establish a kind of engineering cell that can simultaneously secrete dopamine (DA) and GDNF, and to study its possible role in the gene therapy of Parkinson’s disease (PD). Methods: GDNF cDNA with Kozak sequence was cloned and transfected into MN9D cell line secreting DA. The engineered cells were co-cultured with primary dopa glue neurons of rats, and DA neurons were detected by immunohistochemistry. RESULTS: The engineered cells were found to prevent the degeneration of DA neurons and help DA neurons to resist the toxic damage of MPP +. CONCLUSION: This paper, for the first time, combines PD strategy and control strategy to construct MN9D engineering cells. The results indicate that it may have important value in the gene therapy of PD.