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背景:肝硬化时可对脊髓等身体其他组织器官产生严重的影响。目的:应用四氯化碳损伤大鼠肝脏制备肝硬化大鼠模型,探讨肝硬化大鼠脊髓内一氧化氮合酶分布情况。设计:完全随机对照实验。单位:首都医科大学解剖教研室。材料:实验于2002-03/2003-12在首都医科大学解剖教研室完成。20只Wistar雄性大鼠随机分为肝硬化组和正常组,每组10只。方法:肝硬化组经四氯化碳损伤大鼠肝脏制备肝硬化大鼠模型,正常大鼠组不作任何处理。于模型制备后3个月,各组大鼠在麻醉状态下开胸,灌注,固定,取脊髓组织,制备切片,采用还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶组化法及LeicaQ500IW图像分析系统对肝硬化大鼠脊髓—氧化氮合酶阳性细胞进行数量及灰度的测定。主要观察指标:①两组大鼠一氧化氮合酶阳性神经元灰度值。②两组大鼠一氧化氮合酶阳性细胞分布情况。结果:20只大鼠均进入结果分析。①两组大鼠一氧化氮合酶阳性神经元灰度均为60(P>0.05)。②在脊髓灰质区,一氧化氮合酶阳性细胞主要分布在中央管周围即脊髓板层第Ⅹ层和中间外侧核。还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶组化法呈色为强阳性,细胞形态多为三角形和梭形,胞核不着色,胞质色深。细胞中等大小,以25μm为主。在脊髓颈、胸、腰各段灰质中间带一氧化氮合酶阳性细胞分布的数量基本相等,没有特异性变化。结论:肝硬化大鼠与正常大鼠-氧化氮合酶在脊髓内的表达基本相同。一氧化氮在脊髓节段的分布特性可能说明肝硬化大鼠在低级交感神经的调控上与正常无差异。
Background: Cirrhosis can have serious effects on other tissues and organs of the body such as the spinal cord. Objective: To investigate the distribution of nitric oxide synthase in the spinal cord of cirrhotic rats using cirrhotic rat model of liver injury induced by carbon tetrachloride. Design: Complete randomized controlled experiment. Unit: Department of Anatomy, Capital University of Medical Sciences. Materials: The experiment was performed at the Department of Anatomy, Capital Medical University from March 2002 to December 2003. Twenty Wistar male rats were randomly divided into liver cirrhosis group and normal group, 10 rats in each group. Methods: Liver cirrhosis rats were killed by carbon tetrachloride-induced liver cirrhosis rat model, the normal rats without any treatment. At 3 months after model preparation, rats in each group underwent thoracotomy, perfusion and fixation under anesthesia. Spinal cord tissues were obtained and sections were prepared. Using reduced nicotinamide adenine dinucleotide phosphate diaphorase histochemistry and Leica Q500IW Image analysis system for measuring the number and gray scale of spinal cord nitric oxide synthase positive cells in cirrhotic rats. MAIN OUTCOME MEASURES: ① gray value of nitric oxide synthase positive neurons in two groups. ② The distribution of nitric oxide synthase positive cells in two groups of rats. Results: Twenty rats entered the result analysis. ① The gray level of nitric oxide synthase positive neurons in both groups was 60 (P> 0.05). ② in the gray matter area, nitric oxide synthase positive cells are mainly distributed in the central tube around the spinal cord layer Ⅹ layer and the middle lateral nucleus. Reduced nicotinamide adenine dinucleotide phosphate diaphorase histochemical staining strong positive staining, cell morphology mostly triangular and spindle shape, the nucleus is not colored, the cytoplasm color depth. Medium size cells, 25μm-based. In the neck, thoracic and lumbar gray matter in the middle of the nitric oxide synthase positive cells in the distribution of the number of the same, there is no specific change. Conclusion: The expression of nitric oxide synthase in cirrhotic rats and normal rats is basically the same in the spinal cord. The distribution of nitric oxide in the spinal cord segments may indicate that there is no difference in the regulation of lower sympathetic nerve between normal rats and cirrhotic rats.