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【目的】寻找与苦瓜苗期耐低温表达调控相关的蛋白,深入了解苦瓜抗低温机理,为下一步开展苦瓜耐低温分子基础研究及苦瓜耐低温育种打下基础。【方法】以耐冷型MC108和冷敏型MC6苦瓜品种为材料,运用双向电泳技术分析低温胁迫(8℃低温胁迫72 h)下苦瓜苗期叶片蛋白质差异变化。【结果】对2-DE图谱分析发现,冷敏型MC6和耐冷型MC108苦瓜品种间共存在82个差异蛋白点,其中,39个蛋白点上调,36个蛋白点下调,2个新增蛋白点,5个消失蛋白点;对其中24个表达量变化明显的蛋白质点进行MALDI-TOF-TOF/MS分析,经数据库搜索匹配,鉴定出5个未知蛋白和14个功能蛋白,这些蛋白涉及细胞防御、自由基清除、光合作用、基础代谢、蛋白降解等多个功能类别。【结论】在逆境胁迫下苦瓜可通过调节多种蛋白表达来适应外界温度的变化。
【Objective】 The objective of this study is to find out the proteins related to the regulation of low temperature expression at the seedling stage of Momordica charantia L., to understand the mechanism of low temperature resistance of Momordica charantia in deep so as to lay the foundation for the basic research on the molecular mechanism of cryogenic tolerance of Momordica charantia and the low temperature breeding of Momordica charantia. 【Method】 Two-dimensional gel electrophoresis was used to analyze the differences of protein in leaves of bitter melon seedling under cold stress (8 ℃ low temperature stress 72 h), using cold-tolerant MC108 and cold sensitive MC6 bitter gourd varieties as materials. 【Result】 The results of 2-DE analysis showed that there were 82 differential protein spots between the cold-sensitive MC6 and the cold-tolerant MC108 bitter melon, among which 39 were up-regulated, 36 were down-regulated and 2 were newly added , And five disappeared protein spots. MALDI-TOF-TOF / MS analysis was performed on 24 protein spots with obvious changes in expression. Five unknown proteins and 14 functional proteins were identified through database search. These proteins involved in cell defense , Free radical scavenging, photosynthesis, basal metabolism, protein degradation and many other functional categories. 【Conclusion】 Momordica charantia can adapt to the change of external temperature by regulating a variety of protein expression under stress.