有一个学说认为大骨节病是缺硒引起的。亚硒酸钠已在较大范围内被用作一种膳食性预防措施。该病的早期损害是关节软骨和生长板软骨细胞的选择性坏死。本文的实验拟检查在体外可控制的环境下,软骨细胞究竟对硒有无特殊需要;在缺硒的条件下,软骨细胞是否会死亡。本文用家兔的关节软骨和生长板软骨的软骨细胞作单层培养96小时,观察了亚硒酸钠对软骨细胞 DNA 和含硫蛋白多糖合成的影响。我们采用的是一种特殊的培养基(含 DMEM、纤维母细胞生长因子、胰岛素和地塞米松),其含硒量是同位素 There is a doctrine that Kashin-Beck disease is caused by lack of selenium. Sodium selenite has been used as a dietary preventive measure to a large extent. The early damage of the disease is the selective necrosis of articular cartilage and growth plate chondrocytes. In this paper, the experiment to check the in vitro controllable environment, chondrocytes exactly whether the special needs of selenium; in the absence of selenium, the chondrocytes will die. In this paper, rabbit articular cartilage and growth plate cartilage chondrocytes monolayer culture for 96 hours to observe the effect of sodium selenite on chondrocyte DNA and sulfur protein polysaccharide synthesis. We use a special medium (containing DMEM, fibroblast growth factor, insulin and dexamethasone), the selenium content is isotope