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目的研究猴耳环(Pithecellobium clypearia Benth.)的化学成分,并采用DPPH和ABTS法考察分离得到的化合物的体外抗氧化活性。方法采用Sephadex LH-20柱色谱、ODS柱色谱、硅胶柱色谱﹑制备HPLC等分离手段对猴耳环枝叶的化学成分进行分离,通过理化性质及波谱数据进行化合物结构鉴定,采用DPPH和ABTS自由基清除法对分离得到的化合物进行体外抗氧化活性评价。结果从猴耳环的枝叶中分离得到7个单体化合物,分别鉴定为:(+)-lyoniresinol(1)、protocatechuic acid methy ester(2)、(2R,3R)-7,8,3’,4’-tetrahydroxydihydroflavonol(3)、(2R,3S)-5,7,3’-tri-Omethyl-(-)-epicatechin(4)、木犀草素(luteolin,5)、槲皮素(quercetin,6)和没食子酸(gallic acid,7)。结论化合物1~4为首次从猴耳环植物中分离得到。化合物1~7均具有一定的抗氧化活性。
Objective To study the chemical constituents of Pithecellobium clypearia Benth. The DPPH and ABTS methods were used to investigate the antioxidant activity of the isolated compounds. Methods The chemical constituents of the earlobe of the monkey ear were isolated by Sephadex LH-20, ODS, Silica gel column chromatography and preparative HPLC. The structures of the compounds were identified by physico-chemical properties and spectral data. DPPH and ABTS free radical scavenging The isolated compounds were evaluated for anti-oxidative activity in vitro. Results Seven monomeric compounds were isolated from the branches and leaves of monkey earrings and identified as (+) - lyoniresinol (1), protocatechuic acid methy ester (2), (2R, 3R) -7,8,3 ’, 4 (-) - epicatechin (4), luteolin (5), quercetin (6) And gallic acid (7). Conclusion Compounds 1 ~ 4 were isolated from the monkey earring for the first time. Compounds 1 to 7 all have certain antioxidant activity.