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目的研究小肠结肠炎耶尔森菌的耐热性肠毒素B基因(ystB)的分布特征。方法通过聚合酶链反应(PCR)、DNA探针杂交方法与序列分析检测致病性和非致病性小肠结肠炎耶尔森菌携带ystB基因的情况。结果98株非致病性小肠结肠炎耶尔森菌中52株携带ystB基因,占53.06%。全部48株致病性小肠结肠炎耶尔森菌均不携带该基因。结论ystB基因仅存在于部分生物1A型非致病性小肠结肠炎耶尔森菌,而不存在于致病性菌株中。
Objective To study the distribution of heat-stable enterotoxin B (YSTB) gene of Yersinia enterocolitica. Methods The pathogenic and non-pathogenic Yersinia enterocolitica strains carrying ystB gene were detected by polymerase chain reaction (PCR), DNA probe hybridization and sequence analysis. Results Of the 98 strains of Yersinia enterocolitica, 52 strains carried ystB gene, accounting for 53.06%. All 48 pathogenic Yersinia enterocolitica strains did not carry this gene. Conclusion The ystB gene exists only in some species of Yersinia enterocolitica type 1A non-pathogenic, but not in pathogenic strains.