论文部分内容阅读
目的比较不同种属和不同药用部位鬼针草中没食子酸的含量。方法采用RP-HPLC法,用Kromasil C18柱(250mm×4.6 mm,5μm),柱温为25℃,流动相为甲醇-0.1%磷酸水溶液(8:92),检测波长为274 nm,流速为1 ml.min-1。结果没食子酸进样量0.03~0.30μg与峰面积具有良好的线性关系,回归方程为:Y=8.108×104X-3.160×104(r=0.9999,n=6),平均回收率为101.2%。三叶鬼针草根、茎、叶和狼把草、白花鬼针草、婆婆针、三叶鬼针草中没食子酸的含量分别为6.16%、6.95%、9.26%、6.99%、8.5%、9.78%、10.3%。结论所用方法简便、准确、重复性好,可作为控制鬼针草属药材质量的方法;从没食子酸含量的角度考虑,以三叶鬼针草的叶入药最好。
Objective To compare the content of gallic acid in Bidens pilosa of different species and different medicinal sites. Methods The RP-HPLC method was used on a Kromasil C18 column (250mm×4.6mm, 5μm). The column temperature was 25°C. The mobile phase consisted of methanol-0.1% phosphoric acid aqueous solution (8:92). The detection wavelength was 274 nm and the flow rate was 1 Ml.min-1. Results There was a good linear relationship between the injection volume of gallic acid 0.03-0.30 μg and the peak area. The regression equation was: Y=8.108×104X-3.160×104 (r=0.9999, n=6). The average recovery was 101.2%. The contents of gallic acid in the roots, stems and leaves of B. pilosula, wolf’s wolfweed, B. pilosula, L. punctatus and B. pilosula were respectively 6.16%, 6.95%, 9.26%, 6.99%, 8.5%, and 9.78. %, 10.3%. Conclusion The method used is simple, accurate, and reproducible, and can be used as a method for controlling the quality of Bidens medicinal materials. From the perspective of gallic acid content, the leaves of Bidens pilosa is best.