Antibacterial Ag-agents are intensively applied as broad spectrum, high-stability, high-efficiency and high-safety inorganic antibacterial agents. We have developed a new kind of antibacterial Ag-agent, namely Ag_2-x(NH_4)xMo_3O_(10) ·3H_2O nanowires(NWs). Carrying Ag atoms in the lattice and Ag-rich nanoparticles on the surface, the Ag-doped NWs show strong antibacterial effects for a variety of bacteria including E.coli, Staphylococcus aureus, Candida albicans and Aspergil lus niger. By performing systematic comparison experiments, we have proven that the main antibacterial effects are neither resulted from the tiny amount of Ag+ions released from the Ag-doped NWs in aqueous solutions, nor resulted from Ag-rich nanoparticles of fragments of the NWs when they are slowly dissolved in the Martin broth. Instead, the effects are mainly resulted from a contact mechanism, under which, the Ag-doped NWs need to be physically in contact with the bacteria to be eliminated. This is a novel phenomenon observed in the interactions between nanomaterials and live cells, which is worthy of further investigation at the molecular scale. As the Ag-doped NWs are not dissolved in pure water or weak acids, one may find practical antibacterial applications in textile industry and food storage industry for these unique nanomaterials. Antibacterial Ag-agents are intensively applied as broad spectrum, high-stability, high-efficiency and high-safety inorganic antibacterial agents. We have developed a new kind of antibacterial Ag-agents, namely Ag_2-x (NH_4) xMo_3O_ 3H_2O nanowires (NWs). Carrying Ag atoms in the lattice and Ag-rich nanoparticles on the surface, the Ag-doped NWs show strong antibacterial effects for a variety of bacteria including E. coli, Staphylococcus aureus, Candida albicans and Aspergillus lus niger. By performing systematic comparison experiments, we have proven that the main antibacterial effects are are resulted from the tiny amount of Ag + ions released from the Ag-doped NWs in aqueous solutions, nor due from Ag-rich nanoparticles of fragments of the NWs when they Instead, the effects are mainly induced from a contact mechanism, under which, the Ag-doped NWs need to be physically in contact with the bacteria to be eliminated. This is a novel phen As the Ag-doped NWs are not dissolved in pure water or weak acids, one may find practical antibacterial applications in textile industry and food storage industry for these unique nanomaterials.