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Aim:To determine whether the cysteinyl leukotriene receptor 1(CysLT_1 receptor)modulates brain cryoinjury and whether the CysLT_1 receptor antagonist pranlukastexerts a time-dependent protective effect on cryoinjury in mice.Methods:Braincryoinjury was induced by applying a liquid nitrogen-cooled metal probe to thesurface of the skull for 30 s.Brain lesion,neuron density,and endogenous IgGexudation were observed 24 h after cryoinjury.Transcription and the expressionof the CysLT_1 receptor were detected by RT-PCR and immunoblotting,and thelocalization of the receptor protein by double immunofluorescence.Results:ThemRNA and protein expressions of the CysLT_1 receptor were upregulated in thebrain 6-24 h after cryoinjury,and the CysLT_1 receptor protein was primarily local-ized in the neurons,not in the astrocytes or microglia.Pre-injury treatments withmulti-doses and a single dose of pranlukast (0.1 mg/kg) attenuated cryoinjury;postinjury single dose (0.1 mg/kg) at 30 min (not 1 h) after cryoinjury was alsoeffective.Conclusion:The CysLT_1 receptor modulates cryoinjury in mice at leastpartly,and postinjury treatment with its antagonist pranlukast exerts the protec-tive effect with a therapeutic window of 30 min.
Aim: To determine whether the cysteinyl leukotriene receptor 1 (CysLT_1 receptor) modulates brain cryoinjury and whether the CysLT_1 receptor antagonist pranlukastexerts a time-dependent protective effect on cryoinjury in mice. Methods: Braincryoinjury was induced by applying a liquid nitrogen-cooled metal probe to thesurface of the skull for 30 s.Brain lesion, neuron density, and endogenous IgG exudation were observed 24 h after cryoinjury. Transcription and the expression of the CysLT_1 receptor were detected by RT-PCR and immunoblotting, and the localization of the receptor protein by double immunofluorescence. Results: The mRNA and protein expressions of the CysLT_1 receptor were upregulated in the brain 6-24 h after cryoinjury, and the CysLT_1 receptor protein was to be local-ized in the neurons, not in the astrocytes or microglia. Pre-injury treatments with multi-doses and a single dose of pranlukast (0.1 mg / kg) attenuated cryoinjury; postinjury single dose (0.1 mg / kg) at 30 min (not 1 h) after c ryoinjury was alsoeffective.Conclusion: The CysLT_1 receptor modulates cryoinjury in mice at leastpartly, and postinjury treatment with its antagonist pranlukast exerts the protec- tive effect with a therapeutic window of 30 min.