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提出一种以电通量域模式 (吸收信号对电通量作图 )代替以时间域模式 (吸收信号对时间作图 )记录毛细管区带电泳谱图的新模式 .在阐述了这种记录模式优点的基础上 ,用常用的硼砂和磷酸盐体系在不同的电压和固定电压下分离了 4种药物 ,其迁移时间的相对标准偏差分别在 4 1 .5% (不同电压下 )和 1 .90 %以上 (固定电压 ) ,而相应的电通量的标准偏差都在 1 .0 5%以下 ,这进一步从实验角度证实了电通量域模式记录电泳谱图的优点 :具有极强的抗电场强度波动和温度波动的能力 ,提高了再现性 .以电通量模式记录电泳谱图 ,还可在一定程度上提高峰面积的重现性 .这种记录模式降低了对仪器本身的要求 (高压电压电源的稳定性和恒温装置 ) ,有可能促进国产低价位毛细管电泳仪的广泛使用
A new mode of recording electrogram of capillary zone in time-domain mode (absorption signal versus time) is presented in the electric flux domain mode (absorption versus flux plotting). In this paper, The relative standard deviation of the migration time was respectively 4.1% (at different voltages) and 1.90% at different voltages and voltages, respectively % (Fixed voltage), and the standard deviation of the corresponding electric flux are below 1.05%, which further confirms from the experimental point of view that the electric flux domain pattern records the advantages of the electropherogram: has a strong anti-electric field Intensity Fluctuation and Temperature Fluctuation Capability to Improve Reproducibility Electropherogram recordings in flux mode can also improve peak area reproducibility to some extent. This recording mode reduces the need for the instrument itself (high pressure Voltage and power supply stability and thermostat device), it is possible to promote the widespread use of domestic low-cost capillary electrophoresis