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目的研究喉癌混合单抗相关抗原的定位,为喉癌的早期诊断及导向药物治疗奠定理论基础。方法采用三株喉癌单克隆抗体LC9,LC11,LC12混合(MLC)对90例病理证实的喉癌组织切片、14例喉癌前病变及10例正常喉粘膜进行了免疫组化(ABC法)染色并进行光镜观察,同时采用LSAB法对9例新鲜喉癌组织细胞行免疫过氧化物酶标记电镜包埋前染色,对混合单抗相应抗原进行超微结构的定位观察。结果混合单抗检测的相关抗原均分布于细胞膜和/或细胞浆中,细胞核无着色。结论混合单抗相应抗原主要定位于细胞膜性结构,即细胞膜、核膜、线粒体膜和内质网膜
Objective To study the localization of anti-larynx McAb-related antigens in laryngeal carcinoma and lay a theoretical foundation for the early diagnosis and treatment of laryngeal cancer. Methods Totally 90 laryngeal carcinoma sections confirmed by pathology, 14 laryngeal precancerous lesions and 10 normal laryngeal mucosa were examined by immunohistochemistry (ABC method) with three laryngeal cancer monoclonal antibodies LC9, LC11 and LC12 mixed (MLC) Stained and observed with light microscope. At the same time, 9 cases of fresh laryngeal squamous cell carcinoma were stained with immunoperoxidase-labeled electron microscopy by LSAB, and the corresponding antigens of the hybrid mAb were observed by ultrastructure. Results The antigens detected by mixed monoclonal antibodies were all distributed in the cell membrane and / or cytoplasm, and the nuclei were non-staining. CONCLUSIONS: The corresponding antigens of the hybrid mAbs are mainly located in the cell membrane structure, namely the cell membrane, the nuclear membrane, the mitochondrial membrane and the endoplasmic reticulum membrane