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采用在同一切片上,行免疫胶体金染色(IGS)与Grimelius嗜银染色的联合染色方法,对1O只大鼠胃肠道内的内分泌细胞与嗜银细胞进行了对比研究。结果表明,Grimelius嗜银染色法不能显示胃肠道的生长抑素细胞(SS细胞),也基本上不能显示胃泌素细胞(G细胞)。但除胃体外,Grimelius嗜银染色法在胃窦和肠道各段中基本上可用于显示肠嗜铬细胞(EC细胞)。此外,本方法还较其他方法有操作步骤少、结果可靠等优点,可以推广和应用。
Endocrine cells and argyrophilic cells in the gastrointestinal tract of 10 rats were compared using the combined staining method of immunogold gold staining (IGS) and Grimelius silver staining on the same section. The results showed that the Grimelius argyrophilic staining method can not display gastrointestinal somatostatin cells (SS cells), but also basically can not show gastrin cells (G cells). However, in addition to in vitro, Grimelius argyrophil staining can be used essentially to display enterochromaffin cells (EC cells) in the antrum and intestine. In addition, compared with other methods, the method has the advantages of fewer operation steps and reliable results, which can be popularized and applied.