目的　探讨绿脓杆菌MSHA菌苗对肝纤维化大鼠血清肿瘤坏死因子 (TNF)的影响。方法　采用以人血白蛋白攻击方法制备肝纤维化动物模型 ,放免法测定血TNF ,免疫组化法观察肝内层粘蛋白改变。结果　菌苗保护组TNF水平较损伤组、正常对照组明显升高 ,并且保护组肝内层粘蛋白沉积明显减少。结论　菌苗能明显提高肝纤维化大鼠血清TNF水平 Objective To investigate the effect of Pseudomonas aeruginosa MSHA vaccine on serum tumor necrosis factor (TNF) in rats with hepatic fibrosis. Methods The animal model of hepatic fibrosis was established by the method of human serum albumin attack. The blood TNF was measured by radioimmunoassay and the change of mucins in the liver was observed by immunohistochemistry. Results The level of TNF in the vaccine-protected group was significantly higher than that in the injury group and the normal control group, and the deposition of mucin in the protective group was significantly reduced. Conclusions Bacterial vaccine can significantly improve the level of serum TNF in rats with hepatic fibrosis